Fluorescence Characterization of the Transfer RNA-like Domain of Transfer Messenger RNA in Complex with Small Binding Protein B
نویسندگان
چکیده
منابع مشابه
Independent binding sites of small protein B onto transfer-messenger RNA during trans-translation
Stalled bacterial ribosomes are freed by transfer-messenger RNA (tmRNA). With the help of small protein B (SmpB), protein synthesis restarts and tmRNA adds a tag to the stalled protein for destruction. The conformation of a 347 nt long tmRNA from a thermophile and its interactions with SmpB were monitored using structural probes. The RNA is highly folded, including the reading frame, with <30% ...
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tmrna (also known as ssra or 10sa rna) is so-named for its dual nature so that can function like trna and mrna. tmrna involved in a trans-translation reaction that truly is a kind of translational quality control system. this process contributes to the recycling of stalled translation complexes at the end of an mrna lacking a stop codon or at an internal mrna cluster of rare codons in a process...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2012
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi201751k